{"id":936,"date":"2026-08-02T15:12:36","date_gmt":"2026-08-02T15:12:36","guid":{"rendered":"https:\/\/lumexislaser.com\/?page_id=936"},"modified":"2026-08-05T10:47:44","modified_gmt":"2026-08-05T10:47:44","slug":"spectroscopy-fluorescence","status":"publish","type":"page","link":"https:\/\/lumexislaser.com\/es\/solutions\/spectroscopy-fluorescence\/","title":{"rendered":"525 nm Fiber-Coupled Lasers for Spectroscopy and Fluorescence Instruments"},"content":{"rendered":"<style>\nbody.page-id-936{overflow-x:clip}\nbody.page-id-936 .page-header,body.page-id-936 h1.entry-title{display:none!important}\nbody.page-id-936 .lumexis-solution-hero>.wp-block-columns{width:100%!important;max-width:1140px!important;margin-left:auto!important;margin-right:auto!important;align-items:stretch}\nbody.page-id-936 .lumexis-solution-hero .wp-block-column:last-child{display:flex}\nbody.page-id-936 .lumexis-solution-hero .wp-block-column:last-child figure{display:grid;grid-template-rows:minmax(0,1fr) auto;width:100%;height:100%;margin:0}\nbody.page-id-936 .lumexis-solution-hero .wp-block-column:last-child img{width:100%;height:100%;min-height:0;object-fit:cover}\nbody.page-id-936 .lumexis-visual-card{width:100%!important;max-width:1140px!important;margin-left:auto!important;margin-right:auto!important;overflow:hidden;box-shadow:0 14px 34px rgba(17,24,39,.06)}\nbody.page-id-936 .lumexis-visual-card .wp-block-image,body.page-id-936 .lumexis-visual-card figure{margin:0;height:100%}\nbody.page-id-936 .lumexis-visual-card img{width:100%;height:100%;min-height:300px;object-fit:cover}\nbody.page-id-936 .lumexis-solution-table{overflow-x:auto}\nbody.page-id-936 .lumexis-solution-table table{min-width:760px}\n@media(max-width:781px){body.page-id-936 .lumexis-solution-hero .wp-block-column:last-child figure{display:block;height:auto}body.page-id-936 .lumexis-solution-hero .wp-block-column:last-child img,body.page-id-936 .lumexis-visual-card img{height:auto;min-height:0}}\n<\/style>\n\n<div class=\"wp-block-group alignfull lumexis-solution-hero has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#0b0b0d;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<div class=\"wp-block-columns alignwide is-layout-flex wp-container-core-columns-is-layout-bf561b98 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:55%\">\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#c9a24b;margin-bottom:18px;font-size:12px;font-weight:700;letter-spacing:0.12em;text-transform:uppercase\">Espectroscop\u00eda y fluorescencia<\/p>\n\n<h1 class=\"wp-block-heading has-text-color\" style=\"color:#ffffff;margin-bottom:24px;font-size:clamp(28px,3vw,40px);font-weight:600;line-height:1.12\">L\u00e1seres de espectroscop\u00eda: Configuraci\u00f3n seg\u00fan espectro y muestra<\/h1>\n\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#d5d5d2;margin-bottom:28px;font-size:15px;line-height:1.7\">Lumexis suministra 525 nm <a href=\"https:\/\/lumexislaser.com\/es\/fiber-coupled-lasers\/\">l\u00e1seres acoplados a fibra<\/a> para excitaci\u00f3n de fluorescencia compatible y espectroscop\u00eda de longitud de onda fija en instrumentos anal\u00edticos cient\u00edficos, industriales y OEM.<\/p>\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size\" style=\"font-size:13px;font-weight:700\"><a class=\"wp-block-button__link has-text-color has-background wp-element-button\" href=\"https:\/\/lumexislaser.com\/es\/contact\/\" style=\"border-radius:2px;color:#0b0b0d;background-color:#c9a24b\">Discuta su aplicaci\u00f3n con Lumexis<\/a><\/div>\n<\/div><\/div>\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:45%\">\n<figure class=\"wp-block-image size-full\"><img fetchpriority=\"high\" decoding=\"async\" width=\"900\" height=\"600\" src=\"https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-spectroscopy-fluorescence-hero.webp\" alt=\"Instrumento de fluorescencia cerrado que utiliza excitaci\u00f3n verde de 525 nm entregada por fibra y \u00f3ptica de recolecci\u00f3n\" class=\"wp-image-930\" srcset=\"https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-spectroscopy-fluorescence-hero.webp 900w, https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-spectroscopy-fluorescence-hero-300x200.webp 300w, https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-spectroscopy-fluorescence-hero-768x512.webp 768w, https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-spectroscopy-fluorescence-hero-600x400.webp 600w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/figure>\n<\/div><\/div><\/div>\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<p class=\"wp-block-paragraph\">Lumexis suministra <strong>l\u00e1seres de 525 nm acoplados a fibra para fluorescencia<\/strong> excitaci\u00f3n y espectroscop\u00eda de longitud de onda fija en instrumentos anal\u00edticos cient\u00edficos, industriales y OEM compatibles. La entrega por fibra permite a los dise\u00f1adores de equipos colocar la fuente y su interfaz t\u00e9rmica lejos del cabezal de muestra, y luego dirigir la potencia \u00f3ptica verde a una cubeta, microscopio, superficie de material o celda de flujo.<\/p>\n\n<p class=\"wp-block-paragraph\">El 525 nm es \u00fatil solo cuando se superpone con la banda de absorci\u00f3n o excitaci\u00f3n de la muestra, marcador o material que se mide. No es una longitud de onda de fluorescencia universal. Antes de seleccionar la potencia, confirme el espectro de la muestra, la respuesta del detector y el conjunto de filtros. En muchos instrumentos anal\u00edticos, la potencia requerida en la muestra es mucho menor que la salida de clase de vatio publicada de la fuente, por lo que la atenuaci\u00f3n, expansi\u00f3n del haz, divisi\u00f3n o una configuraci\u00f3n de menor potencia deben dise\u00f1arse deliberadamente.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Adapte la fuente a la arquitectura anal\u00edtica<\/h2>\n\n<figure class=\"wp-block-table alignwide is-style-stripes lumexis-solution-table\"><table>\n<thead>\n<tr>\n<th>Arquitectura de medici\u00f3n<\/th>\n<th>Qu\u00e9 contribuye el 525 nm<\/th>\n<th>Entradas de dise\u00f1o cr\u00edticas<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Espectroscop\u00eda de fluorescencia a 90\u00b0<\/td>\n<td>Excitaci\u00f3n verde fija mientras la emisi\u00f3n se recoge lejos del haz directo<\/td>\n<td>Absorci\u00f3n de la muestra a 525 nm, geometr\u00eda de la cubeta, espectro de emisi\u00f3n, filtro de rechazo<\/td>\n<\/tr>\n<tr>\n<td>Im\u00e1genes de materiales por epifluorescencia<\/td>\n<td>Excitaci\u00f3n entregada por fibra a trav\u00e9s de un dicroico y objetivo<\/td>\n<td>Separaci\u00f3n de excitaci\u00f3n\/emisi\u00f3n, uniformidad de campo, transmisi\u00f3n del objetivo, sensibilidad de la c\u00e1mara<\/td>\n<\/tr>\n<tr>\n<td>Interrogaci\u00f3n \u00f3ptica en celda de flujo<\/td>\n<td>Regi\u00f3n de excitaci\u00f3n peque\u00f1a y estable a trav\u00e9s de una corriente l\u00edquida o de part\u00edculas<\/td>\n<td>Cintura del haz, geometr\u00eda del canal de flujo, tiempo de residencia, \u00e1ngulo s\u00f3lido de recolecci\u00f3n y fondo<\/td>\n<\/tr>\n<tr>\n<td>Fluorescencia de superficie o recubrimiento<\/td>\n<td>Punto o l\u00ednea controlada en una superficie de material<\/td>\n<td>Distancia de trabajo, dispersi\u00f3n de superficie, irradiancia de excitaci\u00f3n y \u00e1ngulo de recolecci\u00f3n<\/td>\n<\/tr>\n<tr>\n<td>Fotometr\u00eda de longitud de onda fija<\/td>\n<td>Entrada conocida de 525 nm para transmisi\u00f3n, reflectancia o cambio relativo<\/td>\n<td>Longitud de trayectoria \u00f3ptica, canal de referencia, linealidad del detector y estabilidad de la fuente<\/td>\n<\/tr>\n<tr>\n<td>Plataforma de an\u00e1lisis espectral OEM<\/td>\n<td>Fuente de excitaci\u00f3n para un espectr\u00f3metro de emisi\u00f3n o detector de resoluci\u00f3n de longitud de onda<\/td>\n<td>Rango del espectr\u00f3metro, rechazo de luz dispersa, calibraci\u00f3n y sincronizaci\u00f3n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/figure>\n\n<p class=\"wp-block-paragraph\"><strong>Coincidencia r\u00e1pida:<\/strong> comience con el espectro de absorci\u00f3n de la muestra. Si el 525 nm produce excitaci\u00f3n \u00fatil, defina la potencia en el plano de la muestra y la banda de detecci\u00f3n. Luego seleccione la fibra y el punto de operaci\u00f3n de la fuente. No comience con el vataje m\u00e1s grande disponible.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Qu\u00e9 puede\u2014y qu\u00e9 no puede\u2014medir un l\u00e1ser de 525 nm<\/h2>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Medici\u00f3n de fluorescencia<\/h3>\n\n<p class=\"wp-block-paragraph\">Una muestra absorbe fotones de excitaci\u00f3n y puede emitir parte de esa energ\u00eda a longitudes de onda m\u00e1s largas. La se\u00f1al emitida es a menudo d\u00e9bil en relaci\u00f3n con la luz de excitaci\u00f3n dispersada por la muestra, cubeta, \u00f3ptica y recinto. Por lo tanto, la geometr\u00eda \u00f3ptica y el filtrado importan tanto como la potencia de la fuente.<\/p>\n\n<p class=\"wp-block-paragraph\">La energ\u00eda de un fot\u00f3n de excitaci\u00f3n es:<\/p>\n\n<div class=\"wp-block-group lumexis-formula-card has-background is-layout-constrained wp-container-core-group-is-layout-44c97188 wp-block-group-is-layout-constrained\" style=\"border-left-color:#c9a24b;border-left-width:3px;background-color:#f7f8fa;margin-top:24px;margin-bottom:24px;padding-top:22px;padding-right:26px;padding-bottom:22px;padding-left:26px\">\n<p class=\"has-text-align-center wp-block-paragraph\" style=\"font-size:24px\"><em>E<\/em><sub>fot\u00f3n<\/sub> = <em>hc<\/em> \/ \u03bb<\/p>\n<\/div>\n\n<p class=\"wp-block-paragraph\">A una longitud de onda fija, aumentar la potencia \u00f3ptica aumenta la tasa de llegada de fotones. Esto puede aumentar la se\u00f1al de fluorescencia en un r\u00e9gimen lineal apropiado, pero solo hasta que la saturaci\u00f3n de la muestra, el cambio fotoqu\u00edmico, la saturaci\u00f3n del detector o el calentamiento se vuelvan limitantes.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Espectroscop\u00eda de longitud de onda fija<\/h3>\n\n<p class=\"wp-block-paragraph\">Una fuente de 525 nm proporciona una longitud de onda controlada. Puede soportar experimentos de transmisi\u00f3n, reflectancia, dispersi\u00f3n o emisi\u00f3n a esa longitud de onda de excitaci\u00f3n. No reemplaza una fuente sintonizable o de banda ancha cuando el objetivo es escanear un espectro de absorci\u00f3n a trav\u00e9s de muchas longitudes de onda.<\/p>\n\n<p class=\"wp-block-paragraph\">Cuando un espectr\u00f3metro registra la luz emitida por una muestra excitada a 525 nm, el resultado puede ser un espectro de emisi\u00f3n. Cuando la longitud de onda de la fuente en s\u00ed debe escanearse para producir un espectro de excitaci\u00f3n, un l\u00e1ser fijo de 525 nm suministra solo un punto a menos que el instrumento incluya fuentes adicionales o un elemento sintonizable.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Por qu\u00e9 la entrega por fibra ayuda al dise\u00f1o de instrumentos OEM<\/h2>\n\n<p class=\"wp-block-paragraph\">Una fuente acoplada a fibra separa el empaque del l\u00e1ser del cabezal de medici\u00f3n. Esto puede ayudar al integrador a:<\/p>\n\n<ul class=\"wp-block-list\">\n<li>montar la fuente en un disipador de calor dedicado;<\/li>\n<li>reducir el calor y el ruido el\u00e9ctrico cerca de un detector sensible;<\/li>\n<li>dirigir la luz a una c\u00e1mara de muestra compacta o remota;<\/li>\n<li>exchange collimators, beam expanders or focusing heads;<\/li>\n<li>split or switch optical power between channels with a controlled optical budget;<\/li>\n<li>service the source without disturbing the calibrated sample geometry.<\/li>\n<\/ul>\n\n<p class=\"wp-block-paragraph\">Fiber delivery also introduces design responsibilities. Core diameter and numerical aperture determine the output cone and achievable focus. Connector loss, fiber bend, modal distribution and end-face cleanliness can change delivered power and spatial profile.<\/p>\n\n<div class=\"wp-block-group alignwide lumexis-visual-card has-border-color has-background is-layout-constrained wp-block-group-is-layout-constrained\" style=\"border-color:#dedede;border-width:1px;border-radius:2px;background-color:#ffffff;margin-top:34px;margin-bottom:34px\">\n<div class=\"wp-block-columns are-vertically-aligned-stretch is-layout-flex wp-container-core-columns-is-layout-946c1612 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-stretch is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:58%\">\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" src=\"https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-fiber-laser-fluorescence-integration.webp\" alt=\"525 nm fiber-coupled laser integrated with a cuvette, collection optics and analytical detector\"\/><\/figure>\n<\/div>\n\n<div class=\"wp-block-column is-vertically-aligned-stretch is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:42%\">\n<div class=\"wp-block-group is-layout-constrained wp-container-core-group-is-layout-b2c065c3 wp-block-group-is-layout-constrained\" style=\"padding-top:clamp(28px,4vw,48px);padding-right:clamp(24px,4vw,48px);padding-bottom:clamp(28px,4vw,48px);padding-left:clamp(24px,4vw,48px)\">\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#a17d2f;margin-bottom:12px;font-size:11px;font-weight:700;letter-spacing:0.12em;text-transform:uppercase\">System architecture<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:14px;font-size:clamp(22px,2.4vw,30px);font-weight:650;line-height:1.2\">Review the complete optical and measurement chain<\/h3>\n\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#4c5157;margin-bottom:0;font-size:14px;line-height:1.7\">525 nm fiber-coupled laser integrated with a cuvette, collection optics and analytical detector<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Three practical collection geometries<\/h2>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">1. 90-degree cuvette collection<\/h3>\n\n<p class=\"wp-block-paragraph\">The green beam passes through a transparent sample cell. Fluorescence is collected at approximately 90 degrees to reduce direct transmission into the detector. An emission filter rejects residual 525 nm scatter while the spectrometer or detector measures the longer-wavelength signal.<\/p>\n\n<p class=\"wp-block-paragraph\">This geometry is common in concept because it provides spatial separation between excitation and collection. Its performance depends on sample concentration, optical path length, inner-filter effects, cuvette fluorescence and the collection volume shared by the excitation beam and detector optics.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">2. Epi-fluorescence imaging<\/h3>\n\n<p class=\"wp-block-paragraph\">A dichroic beamsplitter reflects the green excitation through an objective toward the sample. Longer-wavelength emission returns through the same objective and passes through the dichroic and an emission filter to the camera. This arrangement supports compact imaging but places demanding rejection requirements on the filter set because excitation and emission share much of the optical path.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">3. Side collection from a flow cell<\/h3>\n\n<p class=\"wp-block-paragraph\">A focused beam intersects a transparent channel. Collection optics observe fluorescence from the illuminated volume at an angle away from the direct beam. Beam waist, channel dimensions, particle or fluid velocity, detector bandwidth and trigger timing determine signal per event.<\/p>\n\n<div class=\"wp-block-group alignwide lumexis-visual-card has-border-color has-background is-layout-constrained wp-block-group-is-layout-constrained\" style=\"border-color:#dedede;border-width:1px;border-radius:2px;background-color:#ffffff;margin-top:34px;margin-bottom:34px\">\n<div class=\"wp-block-columns are-vertically-aligned-stretch is-layout-flex wp-container-core-columns-is-layout-946c1612 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-stretch is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:58%\">\n<div class=\"wp-block-group is-layout-constrained wp-container-core-group-is-layout-b2c065c3 wp-block-group-is-layout-constrained\" style=\"padding-top:clamp(28px,4vw,48px);padding-right:clamp(24px,4vw,48px);padding-bottom:clamp(28px,4vw,48px);padding-left:clamp(24px,4vw,48px)\">\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#a17d2f;margin-bottom:12px;font-size:11px;font-weight:700;letter-spacing:0.12em;text-transform:uppercase\">System architecture<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:14px;font-size:clamp(22px,2.4vw,30px);font-weight:650;line-height:1.2\">Review the complete optical and measurement chain<\/h3>\n\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#4c5157;margin-bottom:0;font-size:14px;line-height:1.7\">Cuvette, epi-fluorescence and flow-cell architectures using 525 nm excitation<\/p>\n<\/div>\n<\/div>\n\n<div class=\"wp-block-column is-vertically-aligned-stretch is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:42%\">\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" src=\"https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/525nm-fluorescence-instrument-architectures.webp\" alt=\"Cuvette, epi-fluorescence and flow-cell architectures using 525 nm excitation\"\/><\/figure>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Match 525 nm to the sample spectrum<\/h2>\n\n<p class=\"wp-block-paragraph\">Fluorescence excitation is governed by the overlap between source wavelength and the sample\u2019s excitation or absorption spectrum. A manufacturer\u2019s listed excitation maximum is a useful starting point, not a complete compatibility statement.<\/p>\n\n<p class=\"wp-block-paragraph\">Check:<\/p>\n\n<ul class=\"wp-block-list\">\n<li>excitation efficiency specifically at 525 nm;<\/li>\n<li>emission spectrum and separation from 525 nm;<\/li>\n<li>how solvent, binding, concentration, temperature or material state shifts the spectrum;<\/li>\n<li>sample autofluorescence under green illumination;<\/li>\n<li>detector sensitivity across the selected emission band;<\/li>\n<li>optical transmission of the objective, cuvette, fiber and filters.<\/li>\n<\/ul>\n\n<p class=\"wp-block-paragraph\">A marker peaking at a shorter wavelength may still absorb at 525 nm, but less efficiently. Compensating with more power can increase background, photochemical stress or heating. Comparing spectral overlap and measured signal-to-background is more reliable than choosing by peak wavelength alone.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Filter design: reject excitation before increasing power<\/h2>\n\n<p class=\"wp-block-paragraph\">A fluorescence filter set may include an excitation filter, dichroic beamsplitter and emission filter. With a laser source, an additional excitation filter may or may not be required depending on spectral purity and system needs, but the dichroic and emission filter remain central to separating the weak signal from reflected green light.<\/p>\n\n<div class=\"wp-block-group alignwide lumexis-visual-card has-border-color has-background is-layout-constrained wp-block-group-is-layout-constrained\" style=\"border-color:#dedede;border-width:1px;border-radius:2px;background-color:#ffffff;margin-top:34px;margin-bottom:34px\">\n<div class=\"wp-block-columns are-vertically-aligned-stretch is-layout-flex wp-container-core-columns-is-layout-946c1612 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-stretch is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:58%\">\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" src=\"https:\/\/lumexislaser.com\/wp-content\/uploads\/2026\/08\/fluorescence-filter-optical-path.webp\" alt=\"Conceptual 525 nm fluorescence path with dichroic separation and emission filtering\"\/><\/figure>\n<\/div>\n\n<div class=\"wp-block-column is-vertically-aligned-stretch is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:42%\">\n<div class=\"wp-block-group is-layout-constrained wp-container-core-group-is-layout-b2c065c3 wp-block-group-is-layout-constrained\" style=\"padding-top:clamp(28px,4vw,48px);padding-right:clamp(24px,4vw,48px);padding-bottom:clamp(28px,4vw,48px);padding-left:clamp(24px,4vw,48px)\">\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#a17d2f;margin-bottom:12px;font-size:11px;font-weight:700;letter-spacing:0.12em;text-transform:uppercase\">Spectral design<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:14px;font-size:clamp(22px,2.4vw,30px);font-weight:650;line-height:1.2\">Match wavelength, filtering, and detector response<\/h3>\n\n<p class=\"has-text-color wp-block-paragraph\" style=\"color:#4c5157;margin-bottom:0;font-size:14px;line-height:1.7\">Conceptual 525 nm fluorescence path with dichroic separation and emission filtering<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Excitation rejection<\/h3>\n\n<p class=\"wp-block-paragraph\">The emission channel must block the source wavelength over the full range of incidence angles and operating temperature. The relevant specification is optical density or blocking across the actual source spectrum, not only the nominal filter edge.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Emission transmission<\/h3>\n\n<p class=\"wp-block-paragraph\">Choose the passband to capture useful fluorescence while excluding 525 nm leakage, unwanted sample autofluorescence and adjacent channels. A broad passband collects more signal; a narrower band can improve selectivity but discards photons.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Dichroic transition region<\/h3>\n\n<p class=\"wp-block-paragraph\">When excitation and emission bands sit close together, the dichroic transition becomes critical. A larger Stokes shift generally makes optical separation easier. Angle of incidence, polarization and manufacturing tolerance affect the real transition curve.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Stray-light control<\/h3>\n\n<p class=\"wp-block-paragraph\">Blackened baffles, non-reflective mounts, beam dumps and a closed sample chamber reduce light that filters alone cannot manage. Small reflections from a cuvette edge or metal surface can exceed the fluorescence signal.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Source parameters that affect analytical performance<\/h2>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Sample-plane power and irradiance<\/h3>\n\n<p class=\"wp-block-paragraph\">Specify optical power at the sample after fiber, collimator, attenuation and other optics. For an illuminated area (A):<\/p>\n\n<div class=\"wp-block-group lumexis-formula-card has-background is-layout-constrained wp-container-core-group-is-layout-44c97188 wp-block-group-is-layout-constrained\" style=\"border-left-color:#c9a24b;border-left-width:3px;background-color:#f7f8fa;margin-top:24px;margin-bottom:24px;padding-top:22px;padding-right:26px;padding-bottom:22px;padding-left:26px\">\n<p class=\"has-text-align-center wp-block-paragraph\" style=\"font-size:24px\"><em>E<\/em> = <em>P<\/em><sub>sample<\/sub> \/ <em>A<\/em><\/p>\n<\/div>\n\n<p class=\"wp-block-paragraph\">The same power produces very different irradiance when focused to a small spot or expanded over an image field. State spot size or illuminated area with the power value.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Stability and noise<\/h3>\n\n<p class=\"wp-block-paragraph\">Relative measurements can be limited by source fluctuation. Define warm-up time, short-term noise, long-term drift and repeatability after power cycling. A reference photodiode can normalize part of the variation, provided its optical pickoff and detector remain stable.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Linearity and control range<\/h3>\n\n<p class=\"wp-block-paragraph\">If the instrument measures concentration or relative intensity, verify that the source control, sample response and detector remain in a usable linear range. Current adjustment alone may change wavelength, spatial profile or noise. External attenuation can provide an alternative when spectral and spatial consistency is important.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Operating mode and modulation<\/h3>\n\n<p class=\"wp-block-paragraph\">Continuous excitation supports steady-state measurements. Modulation can enable synchronous detection or background subtraction when the driver, source and detector timing are compatible. Define frequency, duty cycle, rise\/fall behavior and trigger reference.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Wavelength tolerance and spectral width<\/h3>\n\n<p class=\"wp-block-paragraph\">The filter and sample should tolerate the source\u2019s actual center wavelength and width across temperature and operating conditions. For a steep sample absorption edge or tight dichroic transition, small wavelength changes can alter signal or leakage.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Fiber core, numerical aperture and output profile<\/h3>\n\n<p class=\"wp-block-paragraph\">The fiber sets the source \u00e9tendue delivered to the sample head. Core diameter and numerical aperture must match the collimator or focusing optic. For imaging illumination, also evaluate spatial uniformity and modal structure; for a focused interrogation spot, evaluate spot size and stability at the real working distance.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Polarizaci\u00f3n<\/h3>\n\n<p class=\"wp-block-paragraph\">If the instrument uses polarization-sensitive samples, dichroics or measurements, define output polarization behavior and how the fiber route affects it. Standard multimode fiber delivery should not be assumed to preserve a fixed polarization state unless the configuration is designed and verified for that purpose.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Thermal integration<\/h3>\n\n<p class=\"wp-block-paragraph\">Watts-class green output requires a deliberate heat path at the source. At the sample, even much lower power can cause local heating or photochemical change when concentrated. Validate temperature and signal behavior over the intended exposure and duty cycle.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Photobleaching, saturation and sample protection<\/h2>\n\n<p class=\"wp-block-paragraph\">More excitation does not always improve data. Excess optical dose can reduce fluorescence over time, change the sample, increase background or saturate the detector.<\/p>\n\n<p class=\"wp-block-paragraph\">Control optical dose through:<\/p>\n\n<ul class=\"wp-block-list\">\n<li>the lowest irradiance that meets signal-to-noise requirements;<\/li>\n<li>shorter exposure or lower duty cycle;<\/li>\n<li>beam expansion for area illumination;<\/li>\n<li>neutral-density attenuation;<\/li>\n<li>triggered excitation only during detector acquisition;<\/li>\n<li>sample movement or flow when appropriate;<\/li>\n<li>acceptance tests across the complete measurement duration.<\/li>\n<\/ul>\n\n<p class=\"wp-block-paragraph\">For process instruments, test both immediate signal and drift over repeated measurements. A stable initial reading can hide dose-dependent change that appears later in the cycle.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Lumexis 525 nm source range for OEM evaluation<\/h2>\n\n<figure class=\"wp-block-table alignwide is-style-stripes lumexis-solution-table\"><table>\n<thead>\n<tr>\n<th>Modelo<\/th>\n<th style=\"text-align: right;\">Published optical power<\/th>\n<th>Practical evaluation note<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>525-LXGX0003<\/td>\n<td style=\"text-align: right;\">2 W<\/td>\n<td>Lowest published family power; sample attenuation may still be required<\/td>\n<\/tr>\n<tr>\n<td>525-LXGX0004<\/td>\n<td style=\"text-align: right;\">4 W<\/td>\n<td>Fixed-wavelength analytical source development<\/td>\n<\/tr>\n<tr>\n<td>525-LXGX0005<\/td>\n<td style=\"text-align: right;\">5 W<\/td>\n<td>Additional optical-loss or splitting budget<\/td>\n<\/tr>\n<tr>\n<td>525-LXGX0015<\/td>\n<td style=\"text-align: right;\">15 W<\/td>\n<td>Multi-channel, expanded-beam or higher-throughput OEM evaluation<\/td>\n<\/tr>\n<tr>\n<td>525-LXGX0020<\/td>\n<td style=\"text-align: right;\">20 W<\/td>\n<td>Larger optical budget with dedicated thermal design<\/td>\n<\/tr>\n<tr>\n<td>525-LXGX0035<\/td>\n<td style=\"text-align: right;\">35 W<\/td>\n<td>Specialized high-power analytical or materials platforms<\/td>\n<\/tr>\n<tr>\n<td>525-LXGX0070<\/td>\n<td style=\"text-align: right;\">70 W<\/td>\n<td>Specialized source integration; not a default fluorescence power level<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/figure>\n\n<p class=\"wp-block-paragraph\">Published power is available at the source output under the specified configuration. It is not a recommendation for power incident on a fluorophore, liquid sample or detector. Many fluorescence systems operate at much lower sample power. The optical train must provide safe, stable attenuation and containment appropriate to the instrument.<\/p>\n\n<p class=\"wp-block-paragraph\">Fiber core, numerical aperture, connector, drive conditions, package and wavelength tolerance should be confirmed against the current order-specific specification.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">OEM integration workflow<\/h2>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Step 1: confirm spectral compatibility<\/h3>\n\n<p class=\"wp-block-paragraph\">Provide excitation\/absorption and emission spectra in the real sample environment. Define the signal band and unwanted backgrounds.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Step 2: select collection geometry<\/h3>\n\n<p class=\"wp-block-paragraph\">Choose 90-degree, epi, transmission, reflection or flow-cell collection. Establish sample volume, working distance and collection numerical aperture.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Step 3: define the power at the sample<\/h3>\n\n<p class=\"wp-block-paragraph\">Measure the minimum power and exposure that achieve the required signal-to-noise ratio. Record beam size, optical losses and sample temperature. Do not scale from source wattage without measuring delivered irradiance.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Step 4: close the filter and detector budget<\/h3>\n\n<p class=\"wp-block-paragraph\">Overlay source spectrum, sample emission, dichroic behavior, emission-filter transmission and detector response. Include angular effects and blocking at 525 nm.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Step 5: select fiber and output optics<\/h3>\n\n<p class=\"wp-block-paragraph\">Match core and numerical aperture to the collimator, beam expander or focus optic. Include connector access, bend management, strain relief and clean handling.<\/p>\n\n<h3 class=\"wp-block-heading\" style=\"margin-top:34px;margin-bottom:16px;font-size:clamp(21px,2.4vw,28px);font-weight:650;line-height:1.25\">Step 6: validate stability and optical dose<\/h3>\n\n<p class=\"wp-block-paragraph\">Test warm-up, drift, repeatability, modulation, photobleaching, sample heating and detector linearity. Use reference materials or internal controls appropriate to the measurement method.<\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Information to send Lumexis<\/h2>\n\n<ul class=\"wp-block-list\">\n<li>sample or material and its excitation\/absorption spectrum;<\/li>\n<li>target emission or detection wavelength band;<\/li>\n<li>sample geometry: cuvette, surface, microscope field or flow cell;<\/li>\n<li>required power and spot\/field size at the sample;<\/li>\n<li>continuous or modulated operating mode;<\/li>\n<li>detector, spectrometer, lens and filter set;<\/li>\n<li>fiber core, numerical aperture, termination and length;<\/li>\n<li>package space, cooling method and ambient range;<\/li>\n<li>stability, monitoring and trigger requirements;<\/li>\n<li>prototype quantity, annual volume and validation schedule.<\/li>\n<\/ul>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n\n<div class=\"wp-block-group lumexis-related has-background is-layout-constrained wp-container-core-group-is-layout-c48c1f9f wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;padding-top:clamp(26px,3.5vw,40px);padding-right:clamp(20px,3.5vw,36px);padding-bottom:clamp(26px,3.5vw,40px);padding-left:clamp(20px,3.5vw,36px)\">\n\n<h2 class=\"wp-block-heading\" style=\"font-size:clamp(24px,2.6vw,32px);font-weight:700;line-height:1.12\">Matched Lumexis laser sources<\/h2>\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:15px;line-height:1.6\">525 nm fiber-coupled excitation for fluorescence and fixed-wavelength spectroscopy.<\/p>\n\n\n<ul class=\"wp-block-list\" style=\"font-size:15px;line-height:1.7\"><li><a href=\"https:\/\/lumexislaser.com\/es\/525nm-fiber-coupled-laser\/\">525 nm fiber coupled lasers \u2014 3.2 W to 70 W<\/a><\/li><li><a href=\"https:\/\/lumexislaser.com\/es\/fiber-coupled-lasers\/\">Fiber coupled lasers \u2014 all wavelengths<\/a><\/li><\/ul>\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:15px;line-height:1.6\">For imaging and inspection use of the same platform see <a href=\"https:\/\/lumexislaser.com\/es\/solutions\/machine-vision\/\">visi\u00f3n artificial<\/a>. To confirm absorption match and sample power, <a href=\"https:\/\/lumexislaser.com\/es\/contact\/\">talk to a Lumexis engineer<\/a>.<\/p>\n\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Preguntas frecuentes<\/h2>\n\n<details class=\"wp-block-details is-layout-flow wp-block-details-is-layout-flow\"><summary>\u00bfEs 525 nm adecuado para cada marcador fluorescente verde?<\/summary>\n<p class=\"wp-block-paragraph\">No. El marcador o material debe absorber suficientemente a 525 nm, y la se\u00f1al emitida debe ser separable de la excitaci\u00f3n dispersada. Verifique los espectros completos de excitaci\u00f3n y emisi\u00f3n en el entorno de muestra previsto.<\/p>\n<\/details>\n\n<details class=\"wp-block-details is-layout-flow wp-block-details-is-layout-flow\"><summary>\u00bfPor qu\u00e9 usar un l\u00e1ser en lugar de un LED?<\/summary>\n<p class=\"wp-block-paragraph\">Un l\u00e1ser puede proporcionar mayor radiancia, una longitud de onda definida y una entrega eficiente por fibra hacia un cabezal \u00f3ptico peque\u00f1o o remoto. Un LED puede ser m\u00e1s adecuado cuando se prefiere una iluminaci\u00f3n amplia y de baja coherencia, una menor densidad de potencia o una estructura de costos m\u00e1s simple. Seleccione seg\u00fan la medici\u00f3n, no por categor\u00eda de fuente.<\/p>\n<\/details>\n\n<details class=\"wp-block-details is-layout-flow wp-block-details-is-layout-flow\"><summary>\u00bfEs 3.2 W la potencia de muestra recomendada?<\/summary>\n<p class=\"wp-block-paragraph\">No. Es la potencia de salida de m\u00f3dulo m\u00e1s baja publicada en esta familia. La potencia de muestra requerida puede ser mucho menor y debe establecerse experimentalmente. Use atenuaci\u00f3n dise\u00f1ada y monitoree la potencia en un plano de muestra definido.<\/p>\n<\/details>\n\n<details class=\"wp-block-details is-layout-flow wp-block-details-is-layout-flow\"><summary>\u00bfEl l\u00e1ser crea un espectro de emisi\u00f3n por s\u00ed mismo?<\/summary>\n<p class=\"wp-block-paragraph\">No. Excita la muestra a 525 nm. Un espectr\u00f3metro o detector resuelto en longitud de onda mide el espectro emitido. Una fuente fija tampoco puede escanear un espectro de excitaci\u00f3n sin longitudes de onda adicionales.<\/p>\n<\/details>\n\n<details class=\"wp-block-details is-layout-flow wp-block-details-is-layout-flow\"><summary>\u00bfC\u00f3mo evito que la luz de 525 nm llegue al detector?<\/summary>\n<p class=\"wp-block-paragraph\">Utilice una geometr\u00eda de recolecci\u00f3n adecuada, separaci\u00f3n dicroica, filtrado de emisi\u00f3n, deflectores y trampas de haz. Verifique el bloqueo con el espectro real de la fuente y los \u00e1ngulos \u00f3pticos. Los datos de filtro por s\u00ed solos no tienen en cuenta la dispersi\u00f3n de cada superficie del instrumento.<\/p>\n<\/details>\n\n<details class=\"wp-block-details is-layout-flow wp-block-details-is-layout-flow\"><summary>\u00bfPuede la fuente ser modulada para detecci\u00f3n lock-in?<\/summary>\n<p class=\"wp-block-paragraph\">Potencialmente, dependiendo de la fuente y el controlador seleccionados. Defina la frecuencia, el ciclo de trabajo, el comportamiento de subida\/bajada y la sincronizaci\u00f3n del disparo para que Lumexis pueda revisar la configuraci\u00f3n operativa adecuada.<\/p>\n<\/details>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#f5f5f3;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Configure the source around the spectrum and sample<\/h2>\n\n<p class=\"wp-block-paragraph\">Send the sample spectra, optical geometry, sample-plane power and detector\/filter details. Lumexis will help match the 525 nm source power class, fiber interface, control method and thermal integration to a testable OEM instrument design.<\/p>\n\n<p class=\"wp-block-paragraph\"><strong>Lumexis \u2014 precision laser sources, engineered for the real world.<\/strong><\/p>\n<\/div>\n\n<div class=\"wp-block-group alignfull has-background is-layout-constrained wp-container-core-group-is-layout-c12f9e37 wp-block-group-is-layout-constrained\" style=\"background-color:#ffffff;margin-top:0;margin-bottom:0;padding-top:72px;padding-right:24px;padding-bottom:72px;padding-left:24px\">\n<h2 class=\"wp-block-heading\" style=\"margin-top:0;margin-bottom:32px;font-size:clamp(30px,4vw,44px);font-weight:700;line-height:1.08\">Technical references<\/h2>\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.nist.gov\/publications\/recommendations-and-guidelines-standardization-fluorescence-spectroscopy\" target=\"_blank\" rel=\"noopener\">NIST: Recommendations and Guidelines for Standardization of Fluorescence Spectroscopy<\/a><\/li>\n<li><a href=\"https:\/\/www.microscopyu.com\/techniques\/fluorescence\/introduction-to-fluorescence-microscopy\" target=\"_blank\" rel=\"noopener\">Nikon MicroscopyU: Introduction to Fluorescence Microscopy<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/ca\/en\/home\/references\/molecular-probes-the-handbook\/technical-notes-and-product-highlights\/selecting-optical-filters-for-fluorescence-microscopy.html\" target=\"_blank\" rel=\"noopener\">Thermo Fisher Scientific: Selecting Optical Filters for Fluorescence Microscopy<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cell-analysis\/fluorophores.html\" target=\"_blank\" rel=\"noopener\">Thermo Fisher Scientific: Fluorophore Selection Guide<\/a><\/li>\n<li><a href=\"https:\/\/www.thorlabs.com\/catalogpages\/V21\/825.pdf\" target=\"_blank\" rel=\"noopener\">Thorlabs: Fluorescence Imaging Filters and Sets<\/a><\/li>\n<\/ul>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Integrate a 525 nm fiber-coupled laser into fluorescence and spectroscopy instruments by absorption match, sample power, filters, stability and fiber optics.<\/p>","protected":false},"author":3,"featured_media":930,"parent":11,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-936","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/pages\/936","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/comments?post=936"}],"version-history":[{"count":5,"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/pages\/936\/revisions"}],"predecessor-version":[{"id":1787,"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/pages\/936\/revisions\/1787"}],"up":[{"embeddable":true,"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/pages\/11"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/media\/930"}],"wp:attachment":[{"href":"https:\/\/lumexislaser.com\/es\/wp-json\/wp\/v2\/media?parent=936"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}